Effect of gamma-irradiated treatment on total phenol content and antioxidant activity in the ethanol extracts from different parts of milk thistle (Silybum marianum)

Articles in Press

Document Type : scientific research article

Authors

1 Assistance professor, Department of Food Hygiene, Faculty of Veterinary medicine, Semnan University, Semnan, Iran

2 Associated Professor of Nuclear Science and Technology Research Institute, Tehran, Iran.

3 Associate professor, Department of Medicinal plant science, Nuclear Agriculture Research School, Nuclear Science and Technology Research Institute, Karaj, Iran.ر

Abstract

In recent years, the use of synthetic antioxidants has been limited due to their toxicity and carcinogenicity. Therefore, studies to find new herbal compounds have been considered in the treatment, reduction and prevention of oxidative effects. At the current study, the ethanolic extract was prepared from the seeds, leaves and stems of milk thistle, Silybum marianum. After irradiation of the extracts with doses of 10, 20 and 30 KGy of gamma ray, Total Phenolic Content (TPC) using Folin-Ciocalteau reagent at 263 nm, the percentage of DPPH and ABTS free radical scavenging activity was investigated. On the results of free radical scavenging DPPH and ABTS tests: Seed extract and treated extracts by dose of 20 KGy gamma ray showed significant increase in TPC and antioxidant activity to leaves and stems and compare to the other doses of gamma rays, respectively (P<0.05). Therefore, on the harmful effects of synthetic antioxidants and the obtained results can be suggested replacing synthetic antioxidants with milk thistle seed in particular. Also, Current results suggested that applying gamma irradiated milk thistle seed extract at a dose of 20 kGy as a functional feed additive for aquatic animals would be a promising strategy for protecting them from oxidative stress and inflammation induced especially by High-fat diet.

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Journal of Utilization and Cultivation of Aquatics

Articles in Press, Accepted Manuscript
Available Online from 30 June 2023

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