The effects of probiotic AQUALASE on growth, survival and hematological parameters of Benni, Mesopotamichthys sharpeyi

Document Type : scientific research article

Authors

1 Department of Aquaculther, Ahvaz Branch, Islamic Azad University, Ahvaz, Iran

2 Ms Graduated of Aquaculture, Ahvaz Branch, , Islamic Azad University, Ahvaz, Iran

3 Department of Aquaculture, Ahvaz Branch, Islamic Azad Universiy, Ahvaz, Iran

Abstract

Yeasts have been used as food additive containing protein and vitamins group B for fish nutrition. The present study was aimed to investigate the effects of probiotic, AQUALASE on growth, immunity and hematological parameters of Benii, Mesopotamichthys sharpeyi. Fish were assigned to 12 experimental tanks (with stocking rate of 30 fish per tank) as 3 experimental treatments with three replicates. The treatments were: T1: 0.05% probiotic, T2: 0.1 % probiotic, T3: 0.15% probiotic. Also, one group fed with probiotic -free diet was considered as control. After 60 feeding with experimental diets, it was recognized that probiotic improves the growth indices including: weight, specific growth rate, daily growth rate, feed and protein efficiency and also decreases feed conversion ratio. In this regard, the best results obtained in fish fed with 0.15 probiotic (T3). Survival rate values showed no significant differences between experimental groups. The values of hemeoglubin, MCH and MCHC increased only in T3. In conclusion, with regard to positive effects of AQUALASE on growth and hematology of Benii, this probiotic can be as a growth enhancer for this species.

Keywords


Abdel-Tawwab, M., Abdel-Rahman, A.M., and Ismael, N.E.M. 2008. Evaluation of commercial live baker's yeast, Saccharomyces cerevisiae as a growth and immunity promoter for fry Nile tilapia, Oreochromis niloticus (L.) challenged in situ with Aeromonas hydrophila. Aquaculture, 280: 185-189. https://doi.org/ 10.1111/ j.1365-2109. 2011.02899.x.
Abdulrahman, N., and Ahmed, V. 2015. Comparative Effect of Probiotic (Saccharomyces Cerevisiae), Prebiotic (Fructooligosaccharide Fos) And Their Combination On Some Differential White Blood Cells In Young Common Carp (Cyprinus Carpio L.), Asian Journal of Science and Technology, 6: 1136-1140. DOI: https://doi.org/ 10.30539/iraqijvm.v40i1.131.
Abumourad, I.M.K., Kenwy, A.M., Ibrahim, T.B., and Hanna, M.W.S.S. 2014. Enterococcus fawcium probiotic as a growth promoter and its impact on the expression of the host innate immune in cultured Oreochromis niloticus. Research journal of Pharmaceutical Biological and Chemical Science, 2: 1747-1761. PMID: 25519527 DOI: 10.3920/BM2014.0052.
Ahire, J.J., Jakkamsetty, C., Kashikar, M.S., Lakshmi, S.G., and Madempudi, R.S. 2021. In Vitro Evaluation of Probiotic Properties of Lactobacillus plantarum UBLP40 Isolated from Traditional Indigenous Fermented Food. Probiotics and antimicrobial proteins, pp. 1-12.
Ahmadi, S., Khodadadi, M., Roomiani, L., and Hakimi Mofrad R. 2014. The embryonic development and formation of Bunnei (Barbus sharpeyi Gunther, 1874). Iranian Sceinctific Fisheries Journal, 22: 4. 1-12.
Ahmadifar, E., Akrami, R., Ghelichi, A., and Zarejabad, A.M. 2011. Effects of different dietary prebiotic inulin levels on blood serum enzymes, hematologic, and biochemical parameters of great sturgeon (Huso huso) juveniles, Comparitive Clinical Pathology, 20: 447-451.
Alishahi, M., Mesbah, M., Namjooyan, F., Sabzevarizadeh, M., and Razi Jalali, M. 2012. Comparison of the effect of
some chemical and plant immune stimuli in Oscar (Astronotus ocellatus). Iranian Journal of Veterinary Medicine, 8: 2. 58-68.
Amenyogbe, E., Chen, G., Wang, Z., Huang, J., Huang, B., and Li, H. 2020. The exploitation of probiotics, prebiotics and synbiotics in aquaculture: present study, limitations and future directions.: a review. Aquaculture Int. 28: 1-25.
Ashourpour, A., Zamini, A., Yazdani Sadati, M.A., and Masouleh float, A. 2011. Effect of Saccharomyces cerevisiae yeast cell wall probiotics on growth indices of Acipensernu diventris. Journal of Aquaculture and Fisheries, 2: 8. 61-53.
Bagenal, T. 1978. Methods for assessmet of fish production in fresh waters, Blackwall Scientific pub. Oxf. London.
Bagni, M., Romano, M.G., Finoia, L., and Abelli, G. 2005. Short and long-term effects of a dietary yeast β-1,3-glucan (Macrogard) and alginic acid (Ergosan) preparation on immune response in sea bass (Dicentrarchus labrax). Fish Shellfish Immunology Journal, 18: 4. 311-325.
Biswas, G., Korenaga, H., Takayama, H., Kono, T., Shimokawa, H., and Sakai, M. 2012. Cytokine responses in the common carp, Cyprinus carpio L. treated with baker's yeast extract. Aquaculture, 356: 169-175.
Cha, J.H., Rahimnejad, S., Yang, S.Y., Kim, K.W., and Lee, K.J. 2013. Evaluations of Bacillus spp. as dietary additives on growth performance, innate immunity and disease resistance of olive flounder (Paralichthys olivaceus) against Streptococcus iniae and as water additives. Aquaculture, 402: 50-57.
Cukrowska, B., Ceregra, A., Maciorkowska, E., Surowska, B., Zegadło-Mylik, M.A., Konopka, E., Trojanowska, I., Zakrzewska, M., Bierła, J.B., Zakrzewski, M., and Kanarek, E., 2021. The Effectiveness of Probiotic Lactobacillus rhamnosus and Lactobacillus casei Strains in Children with Atopic Dermatitis and Cow’s Milk Protein Allergy: A Multicenter, Randomized, Double Blind, Placebo Controlled Study. Nutrients, 13: 4. 1169.
Darafsh, F., Soltani, M., Abdolhay, H.A., and Shamsaei Mehrejan, M. 2020. Efficacy of dietary supplementation of Bacillus licheniformis and Bacillus. subtilis probiotics and Saccharomyces cerevisiae (yeast) on the hematological, immune response, and biochemical features of Persian sturgeon (Acipenser persicus) fingerlings. Iranian Journal of Fisheries Sciences, 19: 4. 2024-2038.
Defaei, S., Falahatkar, B., and Effatpanah, I. 2016. Effects of digestrom P.E.P on growth and some hematological parameters of juveniles Beluga sturgeon (Huso huso). Journal of Fisheries Science and Technology, 5: 1. 83-95.
Eills, A.E. 1997. The leucocyte of fish: A review. Journal of fish Biology, 11: 5. 453-491.
Emtiazjoo, M., Hosseinzadeh Sahafi, H., Zargham, D., Bashti, T., and Razmi, K. 2009. The probiotic effect of Saccharomyces cerevisiae on increasing the survival rate of rainbow trout (Onchorynchus mykiss). Marine Science and Technology Research. 4: 1. 58-66.
Esteban, M.A., Cuesta, A., Ortuno, J., and Meseguer, J. 2001. Immunomodulatory effects of dietary intake of chitin on gilthead sea bream (Sparus aurata L.) innate immune system. Fish and Shellfish Immunology, 11: 303-315.
Etisamipour, M., Zamini, A., and Farokhrooz, M. 2014. Comparsion of Growth Indices, Some Blood ParametersAnd Immune System Among Juvenile Rainbow Trout Fishes (Oncorhynchus Mykiss) Fed Up With Different Levels Of Prebiotic Of Yeast Cell Wall (Saccharomyces Cerevisia). Indian Journal of Fundamental and Applied Life Sciences, 4: 3. 438-448.
Freitas, M., Axelsson, L.G., Cayuela, C., Midtvedt, T., and Trugnan, G. 2005. Indigenous microbes and their soluble factors differentially modulate intestinal glycosylation steps in vivo. Use of a "lectin assay" to survey in vivo glycosylation changes. Histochem. Cell. Biol. 124: 423-433.
Gephart, J.A., Golden, C.D., Asche, F., Belton, B., Brugere, C., Froehlich, H.E., Fry, J.P., Halpern, B.S., Hicks, C.C., Jones, R.C., and Klinger, D.H. 2020. Scenarios for global aquaculture and its role in human nutrition. Reviews in Fisheries Science and Aquaculture,3: 1-7.
Ghobadi, Sh., Tavakoli, H., and Majazi Amiri, B. 2014. Effect of different levels of Bactocell probiotic on some growth indices, survival and body composition of common carp (Cyprinus carpio). Journal of Aquaculture Development,
8: 4. 87-77.
Giri, S.S., Sukumaran, V., Sen, S.S., and Jena, P.K. 2014. Effects of dietary supplementation of potential probiotic Bacillus subtilis VSG 1 singularly or in combination with Lactobacillus plantarum VSG 3 or/and Pseudomonas aeruginosa VSG 2 on the growth, immunity and disease resistance of Labeo rohita. Aquaculture Nutrition, 20: 2. 163-171.
Gobi, N., et al. Dietary supplementation of probiotic Bacillus licheniformis Dahb1 improves growth performance, mucus and serum immune parameters, antioxidant enzyme activity as well as resistance against Aeromonas hydrophila in tilapia Oreochromis mossambicus. Fish & Shellfish Immunology,74: 501-508.
Haghighi, M. 2009. Laboratory methods of fish hematology. Aquatic Science Publications. 83p.
Hassanpour Fattahi, A., Jafarian, H., Khosravi, A., and Pourkenani, H. 2014. Effect of Saccharomyces cerevisiae and Aspergillus niger yeast isolated from the digestive tract of adult elephants on the nutritional efficiency and serum enzymes of young elephantfish (Huso huso). Journal of Fisheries Science and Technology, 3: 1. 1-13.
Havixbeck, J.J., Rieger, A.M., Wong, M.E., Hodgkinson, J.W., and Barreda, D.R. 2015. Neutrophil contributions to the induction and regulation of the acute inflammatory response in teleost fish. Journal Leukocyte. Biology.
Hoseini Far, S.H., Mirvaghefi, A., and Mojazi Amiri, B. 2011. Khoshbavar Rostami, H. and Merrifield, D, The effects of Oligofructose on growth performance, survival, intestinal microbiota and liver histology of endangered great sturgeon (Huso huso) juvenile. Aquaculture Nutrition, 17: 5. 498-504.
Irianto, A., and Austin, B. 2002. Probiotic in aquaculture. Journal of Fish Diseases, 25: 1-10.
Kafilzadeh, R., Mousavi, S.M., and Javaheri Baboli, M. 2013. Effects of Saccharomyces cerevisiae (Saccharomycetes: Saccharomycetaceae) on Astronotus ocellatus as growth promoter and immuno stimulant. Aquaculture, Aquarium, Conservation and Legislation International. Journal of the Bioflux Society, 6: 6. 587-598.
Khodadadi, M., Ahmadi, S., and Dezfoulyian, A. 2011. Morphological changes of Bunni (Barbus sharpeyi) larvae in laboratory conditions. Iranian Sceinctific Fisheries Journal, 20: 3. 173-178.
Knipe, H., Temperton, B., Lange, A., Bass, D., and Tyler, C.R. 2021. Probiotics and competitive exclusion of pathogens in shrimp aquaculture. Reviews in Aquaculture, 13: 1. 324-352.
Kumprechtova, D., Zobac, P., and Kumprecht, I. 2000. The effect of Saccharomyces cervisiae Sc47 on chicken broiler performance and nitrogen output. Journal of Animal Science, 12: 45. 169-177.
Mohammad Nejad Shamushki, M., and Maini, M. 2012. The effect of bakery yeast probiotic (Saccharomyces cerevisiae) on the growth and survival of Rutilus rutilus caspicus. Journal of Aquaculture Development, 6: 1. 103-111.
Olsen, R.E., Myklebust, R., Kryvi, H., Mayhew, T.M., and Ringø, E. 2001. Damaging effect of dietary inulin on intestinal enterocytes in Arctic charr (Salvelinus alpinus L.). Aquaculture Research, 32: 11. 931-934.
Pakbin, B., Pishkhan Dibazar, S., Allahyari, S., Javadi, M., Farasat, A., and Darzi, S. 2021. Probiotic Saccharomyces cerevisiae var. boulardii supernatant inhibits survivin gene expression and induces apoptosis in human gastric cancer cells. Food Science & Nutrition, 9: 2. 692-700.
Rahman, A., Shefat, S.H.T., and Chowdhury, M.A. 2021. Effects of Probiotic Bacillus on Growth Performance, Immune Response and Disease Resistance in Aquaculture. Preprints, 2021030075.
Rayes, A.H. 2013. Study on the effect of dietary probiotic bacteria Arthrobacter species, β-1,3 glucan and Moringaoleifera leaf on protection of Penaeus indicus Juveniles from pathogenic Vibrio harveyi. Researcher, 5: 1. 24-31.
Ridha, M.T., and Azad, I.S. 2012. Preliminary evaluation of growth performance and immune response of Nile tilapia Oreochromis niloticus supplemented with two putative probiotic bacteria. Aquaculture Research, 43: 843852. https://doi.org/10.1111/ j.1365-2109.2011.02899.x.
Roque Joel, C., Vera Cruz Emmanuel, C., and Reyes Alvin, T. 2020. Growth, length-weight relationship and condition factor of Nile tilapia (Oreochromis niloticus L.) fed with probiotic bacterium (Bacillus amyloliquifaciens) supplemented diet. International Journal of Fisheries and Aquatic Research, 5: 1. 34-38.
Rufchaie, R., Hoseinifar, S., Sayad Borani, M., Maghsodie Kohan, H., Zamini, A., and Faeed, M. 2012. The effects of glucan on hematological parameters, immune response and intestinal microbiota of Rutilus frisii kutum fry. Iranian Sceinctific Fisheries Journal, 21: 3. 73-84.
Sampantamit, T., Ho, L., Lachat, C., Sutummawong, N., Sorgeloos, P., and Goethals, P. 2020. Aquaculture production and its environmental sustainability in Thailand: challenges and potential solutions. Sustainability, 12(5), 2010. https://doi.org/ 10.3390/ su12052010.
Saputra, F., Shiu, Y.L., Chen, Y.C., Puspitasari, A.W., Danata, R.H., Liu, C.H., and Hu, S.Y. 2016. Dietary supplementation with xylanase-expressing B. amyloliquefaciens R8 improves growth performance and enhances immunity against Aeromonas hydrophila in Nile tilapia (Oreochromis niloticus). Fish & shellfish immunology, 58: 397-405. DOI: 10.1016/ j.fsi. 2016. 09.046.
Schley, P., and Field, C. 2002. The immune enhancing effects of dietary fibers and prebiotics. British Journal
of Nutrition, 87: 2. 221-230. DOI: 10.1079/BJNBJN/2002541.
Sutthi, N., and Thaimuangphol, W. 2020. Effects of yeast (Saccharomyces cerevisiae) on growth performances, body composition and blood chemistry of Nile tilapia (Oreochromis niloticus Linnaeus, 1758) under different salinity conditions. Iranian Journal of Fisheries Sciences, 19: 3. 1428-1446. DOI: 10.22092/ IJFS.2019.119254
Swain, P.S., Dash, P.K., Sahoo, P., Routray, S.K., Sahoo, S.D., Gupta, P.K., and Meher, N. 2006. Nonspecific immune parameters of brood Indian major carp Labeo rohita and their seasonal variations. Fish and Shellfish Immunology, 22: 38-43. DOI: 10. 1016/ j.fsi.2006.03.010
Tewary, A., and Patra, B. 2011. Oral administration of bakers yeast (Saccharomyces cerevisiae) acts as a growth promoter ans immunomodulator in Labeo rohita. Journal of Aquaculture Research and Development, 2: 109. DOI:10.4172/2155-9546.1000109.
Thrall, M.A. 2004. Veterinary Hematology and Clinical Chemistry, Lppincott Williams & Wilkins, USA, pp: 241, 277-288, 402.
Van Doan, H., Hoseinifar, S.H., Khanongnuch, C., Kanpiengjai, A., Unban, K., and Srichaiyo, S. 2018. Host-associated probiotics boosted mucosal and serum immunity, disease resistance and growth performance of Nile tilapia (Oreochromis niloticus). Aquaculture, 491: 94-100. DOI:10.1016/ j.aquaculture.2018.03.019.
Verschuere, L., Dhont, J., Sorgeloos, P., and Verstraete, W. 2000. Probiotic bacteria as biological control agents in aquaculture. Microbiology and Molecular Biology Reviews, 64: 4. 655-671. doi: 10.1128/mmbr.64.4.655-671.2000.
Wang, Y.B., Tian, Z.O., Yao, J.T., and Li, W. 2008. Effect of probiotics, Enteroccus faecium, on tilapia (Oreochromis niloticus) growth performance and immune response. Aquaculture, 277: 203-207.
Williams, B., Verstegen, M.W.A., and Tamminga, S. 2001. Fermentation in the large intestine of single-stomached animals and its relationship to animal health. Nutrition Research Reviews, 14: 2. 207-227. DOI: 10.1079/ NRR200127.
Yarahmadi, B., Mohamadi Saei, M., and Mehrabi, F. 2021. Effect of probiotic (Bacilact) on growth performance,
feed restriction and compensatory growth after re-feeding in rainbow trout. Journal of Aquaculture Development, 14: 4. 119-132.
Yegane, H., Kazemi, R., Shenavar Masoule, A., Sayed Hassani, H., Yousefi Jourdehi, A., Ali hosseinpour Zelti, A., and Ghorbani Vagheyei, R. 2021. The effect of native probiotic on growth rate and some of the hematological and immune system parameters of Huso huso fingerling. Journal of Aquaculture Development, 15: 1. 1-13.
Yuan, C., Li, D., Chen, W., and Sun, F. 2007. Administration of a herbal immunoregulation mixture enhance some immune parameters in carp (Cyprinus carpio). Journal of Fish Physiology and Biochemistery, 10: 1007-1120. DOI: 10.1007/s10695-006-9120-7.