Effect of Spriulina platensis powder on digestion and liver function index enzymes and biochemical parameters in Mugil cephalus Linnaeus, 1758

Document Type : scientific research article

Authors

Chabahar Maritime University

Abstract

This experiment was conducted to evaluate the effect of Spirulina pletensis powder on the liver and digestive enzymes and biochemical parametese of Mugil cephalus for 60 days. The experiment was conducted in a completely randomized design with 450 of grey mullet larvae (with average weight of 0.72±0.02g) in 5 treatments and 3 replicates (n=30 in each replicate) and included: control group without using algae extract, an another groups (treatment 2, 3,4 and 4) the amounts of this extract were 5,10,15 and 30 g/kg food The results showed that at the end of experiment, although the highest the activity of protease and lipase enzymes, the lowest the activity of alanin aminotransferase (ALT) and alkaline phosphates (ALP) were observed in treatment containing 15g/kg spirulina algae powder, in all of these parameters, treatment 5 (20 g/kg) (4) showed a significant difference compared with control treatment (p < 0.05), in ALT, amylase and lipase, there were no significant difference among treatment 4 and 5 (P>0.05). The lowest cholesterol, glucose and triglyceride, the highest globulin, albumon and total protein were recorded in treatment 4. Finally, the present results showed that use of the diet containing 115g/kg spirulina algae had positive effects on the activity of liver and digestive enzymes and blood biochemical parameters of grey mullet.

Keywords


1.Abdel-Daim, M.M., Abuzead, S.M., and Halawa, S.M. 2013. Protective role of Spirulina platensisagainst acute deltamethrin-induced toxicity in rats. PLoS One, 8: 9. 7288-72991.
2.Abdel-Daim, M., El-Baily, B.E., Rahman, H.G., Radi, A.M., Hefny, H.A., and Hassan, A.M. 2016. Antagonistic effects of Spirulina platensis against sub- acute deltamethrin toxicity in mice; biochemical and histopathological studies. Biomedical Pharmacotherapy. 77: 79-85.
3.Ahmadzade-Nia, Y., Adl, K., Hezave, S., Hejazi, M., Hassanpour, S., Chaichisemsari, M., and Riyazi, S. 2011. Effect of replacing different levels of Soybean meal with Spirulina on performance in Rainbow Trout. Annual Biological Research, 2: 3. 374-379.
4.Akbary, P., and Malek Raeisi, E. 2018. Optimum dietary level of Chlorella vulgaris powder as a feed additive for some blood parameters and digestive enzymatic activities of grey mullet, Mugil cephalus. Oceanograph. Fish Open Access J. 7: 5. 1-5.
5.Ali, E.A., Barakat, B.M., and Hassan, R. 2015. Antioxidant and angiostatic effect of Spirulina platensissuspension in complete Freund’s adjuvant- induced arthritis in rats. PLoS One, 10: 4. 0121448-0121523.
6.Ansarifard, F., Rajabi Islami, H., Shamsaie Mehrjan, M., and Soltani, M. 2018. Effects of Arthrospira platensis on growth, skin color and digestive enzymes of Koi, Cyprinus carpio. Iran. J. Fish. Sci. 17: 2. 381-393.
7.Bai, S.D. 2016. Effect of dietary supplementary feed of spirulina on digestive enzymes in fingerlings of common carp (Cyprinus carpio, L. 1758). Europ. J. Pharmaceut. Med. Res.3: 8. 421-426.            
8.Banaee, M., Sureda, A., Mirvaghefi, A.R. and Rafei, G.R. 2011. Effects of long-term silymarin oral supplementation on the blood biochemical profile of rainbow trout (Oncorhynchus mykiss). Fish Physiology and Biochemistry, 37: 887-896.
9.El-Baz, F., Aly, H.F., El-Sayed, A.B.,and Mohamad, A.A. 2013. Role of Spirulina platensis in the control of glycemia in DM2 rats. Int. J. Sci. Engin. Res. 4: 1731-1740.
10.El-Tantawy, W.H., and El-Tantawy, W.H. 2016. Antioxidant effects of Spirulina supplement against lead acetate-induced hepatic injury in rats.J. Traditional and Complementary Medicine, 6: 4. 327-331.
11.Francis, G., Makkar, H.P.S., and Becker, K. 2001. Antinutritional factors present in plant-derived alternate fish feed ingredients and their effects in fish. Aquaculture. 199: 197-227.
12.Ghazi, S., Habibiyan, M., Moeini, M.M., and Abdolmohammadi, A.R. 2012. Effects of dietary selenium, vitamins E, and their combonition on growth, serum metabolites and antioxidant defense system in skeletal muscle of broilers under heat stress. Biological Elementary Research, 148: 322-330.
13.Hoseini, S.M., Khosravi-Darani, K.,and Mozafari, M.R. 2013. Nutritional and medical applications of spirulina microalgae. Mini-Reviews in Medicinal Chemistry, 13: 8. 1231-1237.
14.Khani, M., Soltani, M., Shamsaie Mehrjan, M., Foroudi, F., and Ghaeni, M. 2017. The effects of Chlorella vulgaris supplementation on growth performance, blood characteristics and digestive enzymes in Koi (Cyprinus carpio). Iran. J. Fish. Sci. 16: 2. 832-843.
15.Kurd, F., and Samavati, V. 2015.Water soluble polysaccharides from pirulinaplatensis: extraction and in vitro anti-cancer activity. Inter. J. Biol. Macromol. 74: 498-506.
16.Lermen, C.L., Lappe, R., Crestani, M., Vieira, V.P., Gioda, C.R., Schetinger, M.R.C., Baldisserotto, B., Moraes, G., and Morsch, V.M. 2004. Effect of different temperatureregimes on metabolic and blood parameters of silver catfish Rhamdia quelen. Aquaculture. 239: 497-507.
17.Ma, Q.Y., Fang, M., Zheng, J.H., Ren, D.F., and Lu, J. 2016. Optimised extraction of beta-carotene from Spirulina platensis and hypoglycaemic effect in streptozotocin-induced diabetic mice.J. Sci. Food Agric. 96: 5. 1783-1789.
18.Madibana, M.J., Mlambo, V., Lewis,B., and Fouché, C. 2017. Effect of graded levels of dietary seaweed (Ulvasp.) on grow hematological and serum biochemical parameters in dusky Argyrosomus japonicus, sciaenidae. Egypt. J. Aqua. Res. 107: 1-5.
19.Mukherjee, S., Parial, D., Khatoon,N., Chaudhuri, A., Senroy, S., Homechaudhuri, S., and Pal, R. 2011. Effect of Formulated Algal Diet on growth performance of Labeo rohita Hamilton. J. Algal. Biom. Utilization.2: 4. 1-9.
20.Mukesh, M.A. 2015. Effects of diet substitution on growth performance, energy consumption and digestive enzymes in Macrobrachium rosenbergii postlarvae. Advances in Aquaculture and Fisheries Management, 3: 5. 241-248.
 21.Mustafa, M.D.G., and Nakagawa, H. 1995. A review: dietary benefits of algae as an additive in fish feed. Israeli J. Aquacul. - Bamidgeh, 47: 155-162.
22.Nandeesha, M.C., Gangadhar, B., Varghese, T.J., and Keshavanath, P. 1998. Effect of feeding Spirulina platensis on the growth, proximate composition and organoleptic quality of common carp, Cyprinus carpioL. Aquaculture Research, 29: 305-312.
23.Nya, E.J., and Austin, B. 2009. Useof dietary ginger, Zingiber officinale Roscoe, as an immunostimulant to control Aeromonas hydrophila infections in rainbow trout, Oncorhynchus mykiss (Walbaum). J. Fish Dis. 32: 971-977.
24.Oliveira, M.N., Ponte-Freitas, A.L., Urano-Carvalho, A.F., Taveres-Sampaio, T.M., Farias, D.F., Alves-Teixera, D.I., Gouveia, S.T., Gomes-Pereira, J., and Castro-Catanho de Sena, M.M. 2009. Nutritive and non-nutritive attributes of washed-up seaweeds from the coast of Ceara. Brazilian. Food Chemistry, 11: 254-259.
25.Radhakrishnan, S., Ibrahim, E.H., Belal, C., Seenivasan, T., Muralisankarb, P., and Bhavan, B. 2016. Impact of fishmeal replacement with Arthrospira platensis on growth performance, body composition and digestive enzyme activities of the freshwater prawn, Macrobrachium rosenbergii. Aquaculture Reports, 3: 35-44.
26.Riche, M. 2007. Analysis of refractometry for determining total plasma protein in hybrid striped bass (Morone chrysops × M. saxatilis)at various salinities. Aquaculture.264: 279-284.
27.Sandhu, G.S. 2005. A Textbook ofFish and Fisheries, Dominant Publishers and Distributors, New Delhi, India.Pp: 39-45.
28.Schaperclaus, W., Kulow, H., and schreckenbach, K. 1992. Fish diseases. A.A. Balkema, Rotterdam, the Netherlands.
29.Sherif, A.H., EL-Sheekh, M.M., and Abdel-Halim, S.S. 2012. Effect of Spirulina algae on the health status and growth performance of Nile Tilapia (Oreochromis niloticus) cultured at Kafr El-Sheikh governorate. Egypt J. Basic Appl. Physiol. 11: 1. 57-68.
30.Upasani, C.D., and Balaraman, R.2003. Protective effect of Spirulinaon leadinduced deleterious changes
in the lipid peroxidation and endogenous antioxidants in rats. Phototherapy Research, 17: 330-334.
31.Xu, W., Gao, Z., Qi, Z., Qiu, M., Peng, J., and Shao, R. 2014. Effect of dietary chlorella on the growth performance and physiological parameters of Gibel carp Carassius auratus gibelio. Turk. J. Fish. Aqua. Sci. 14: 53-57.
32.Yong, T.C., Galaz, G.B., and Shapawi, R. 2017. Effects of dietary inclusion of Spirulina meal on growth and hematological parameters of cultured Asian sea bass, Lates calcarifer. Borneo J. Mar. Sci. Aquacul. 1: 1-6.
33.Yu, J., Starr, D.A., Wu, X., Parkhurst, S.M., Zhuang, Y., Xu, R., and Han, M. 2006. The KASH domain protein MSP-300 plays an essential role in nuclear anchoring during Drosophila oogenesis. Developmental Biology, 289: 2. 336-345.
34.Zeinab, A.K., Aly, M.S., Faiza, A.K., and Fatma, E. M.2015. Effect of Spirulina platensisand Lactobacillus rhamnosuson growth and biochemical performance of Nile Tilapia (Oreochromis niloticus) fingerlings. Inter. J. Curr. Microbiol. Appl. Sci.
4: 4. 747-763.