Haematological study of safflower (Carthamus tinctorius) extract fed common carp (Cyprinus carpio) fingerlings exposed to sub lethal salinity stress

Document Type : scientific research article

Author

Gorgan University of Agricultural Sciences and Natural Resources

Abstract

In this study, effect of dietary safflower (Carthamus tinctorius) extract on hematological parameters of common carp (Cyprinus carpio) fingerlings in salinity sub-lethal stress was investigated. In this regard, fish with average weight of 9.51±0.13 g were fed with different levels of safflower extract (0 (control), 0.5, 1.5 and 3 % in diet) for 70 days. At the end of rearing period, the fish were exposed to a sub lethal salinity stress and for hematological indices were studied in four times including before salinity stress, 6, 12 and 48 h after salinity stress. According to the results red blood cell count (RBC) was significantly higher in fish of control group compare with treatment groups, and white blood cells (WBC) was recorded lower in fish of control group compare to treatments (P ˂ 0.05). In all of studied fish levels of RBC, WBC, hematocrit (Hct) and hemoglobin (Hb) increased 6 h post challenge. Red blood cell counts (RBC) increase 24 h and 48 h after salinity stress but other mentioned factors decrease during 48 h post challenge. On the other hand, levels of hemoglobin 48 h after salinity stress in fish that fed with 1.5% and 3% safflower extract in diets were significantly higher compared to other groups and WBC in these fish were recorded lower compare to others 48 h post challenge too (P ˂ 0.05).

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