The effects of Natuzyme multi-enzyme in common carp (Cyprinus carpio) diets on susceptibility to Abamectin exposure

Document Type : scientific research article

Abstract

Some pesticides, such as Abamectin have ability to penetrate and absorbed the skin or digestive system. So, using a variety of multi-enzymes for increasing the efficiency of the digestive system of fish has been increased. The aim of this study was utilizing Natuzyme multi-enzyme in common carp (Cyprinus carpio) diets and investigation of its impact on fish survival rates along with the Abamectin. For this purpose, fishes were divided into 2 treatments with 3 replications and each groups feeding with one type diets for eight weeks. After that time, 21 fishes were exposed from each group with concentrations of 1.243 mg per liter of Abamectin for 96 hours. All of the physicochemical parameters of water and the amount feeding in the two groups were similar in this study and just concentrations of multi-enzymes were related. Finally, LC50 of Abamectin for fishes that they had consumed Natuzyme multi-Enzyme was determined; for this aim 147 fishes exposed in different Concentrations of Abamectin (0.25, 0.5, 1, 2, 3, 6 ml/L) for 96 hour and. mortality rates were recorded at time of 0, 24, 48, 72 and 96 h. Analysis of the data showed significant difference between treatment and control groups in terms of fatalities (P <0.05). Higher mortality related to treatment 1. The 96 h LC50 of abamectin for Treatment 1 was 0.305 mg/L. The results of this study showed that the usage of Natuzyme multi-enzyme in the common carp diets can increase the death rate and toxicity of Abamectin.

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1. Adilian, M. Taghizadeh, Imanpour, M. Mazandarani, M. 2013. The use of
Natuzyme multi-enzyme in common crap (Cyprinus carpio) diet and its effect
on gonadosomatic index. Second National Conference on Fisheries and
Aquaculture, Iran.
2. Alabaster, J.S., and Lloyd, R. 1982. Water Quality Criteria for Freshwater Fish.
Second edition. Butterworth Scientific, London, England. 359p.
3. Bela, Z., and Prasad, R. 2008. Impact of pollution on fresh and marine water
resources. Journal of Pollution Research, 273: 461-466.
4. Bogut, I., Opačak, A., and Stević, I. 1995. “The influence of poly-enzymes
added to the food on the growth of carp fingerlings (Cyprinus carpio L).”
Aquaculture 129(1): 252.
5. Ghomi, M.R., Shahriari R., Faghani H., Nikoo M., and Elert E.V. 2012. Effects
of exogenous dietary enzyme on growth, body composition, and fatty acid
profiles of cultured great sturgeon Huso huso fingerlings. Aquaculture
International, Vol. 20(2): 249–254.
6. Hedayati A., Forouhar M., Mohamadi A., Abarghoei S., and Hajiahmadyan M.
2014. Acute toxicity test of pesticide abamectin on common carp (Cyprinus
carpio). Journal of Coastal Life Medicine, 2(11): 841-844.
7. Hidalgo, M.C., Urea, E., and Sanz, A. 1999. “Comparative study of digestive
enzymes in fish with different nutritional habits. Proteolytic and amylase
activities.” Aquaculture 170(3): 267–283.
8. Lin, Shimei, Kangsen Mai, and Beiping Tan. 2007. “Effects of exogenous
enzyme supplementation in diets on growth and feed utilization in tilapia,
Oreochromis niloticus x O. aureus.” Aquaculture research. 38(15): 1645–1653.
9. Lumaret, J.P., and Errouissi, F. 2002. Use of anthelmintic in herbivores and
evaluation of risks for the non-target fauna of pastures. Veterinary Research,
Vol. 33: 547–562.
10.Mazandarani, M., Taghizadeh, A., Adelian, M. and Imanpour, M. 2009. The use
of Natuzyme multi-enzyme in common carp (Cyprinus carpio) diet and its
effect on gonadosomatic index. Second National Conference on Fisheries and
Aquaculture in Iran, Islamic Azad University, Bandar Abbas Branch, Iran.
11.McKellar, QA, and Benchaoui, HA. 1996. Avermectins and milbemycins.
Journal of Veterinary Pharmacology and Therapeutics, 19: 331-351.
12.Moore, JC., DeRuiter, PC., Hunt, HW. 1993. Soil invertebrate/
microinvertebrate interactions: disproportionate effects of species on food web
structure and function. Veterinary Parasitology, 48: 247–260.
13.Moran, Edwin, T., and James, McGinnis, 1968. “Growth of chicks and turkey
poults fed western barley and corn grain-based rations: Effect of autoclaving on
supplemental enzyme requirement and asymmetry of antibiotic response
between grains.” Poultry Science 47(1): 152–158.
14.Nazifi, S., Firoozbakhsh, F., and Bolouki, M. 2000. Evaluation of serum
biochemichal parameters in experimental intoxication with trichlorofon in silver
carp (Hypophthalmichthys molitrix Valencrennes). Journal of the Faculty of
Veterinary Medicine, 55: 55-60.
15.Novelli, A., Vieira, B.H., Cordeiro, D., Cappelini, L.T.D., Vieira, E.M., and
Espíndola, E.L.G. 2011. Lethal effects of abamectin on the aquatic organisms
Daphnia similis, Chironomus xanthus and Danio rerio. Journal of
Chemosphere, Accepted 28 August 2011.
16.Pettersson, D., and Åman, P. 1989. “Enzyme supplementation of a poultry diet
containing rye and wheat.” British journal of Nutrition. 62(01): 139–149.
17.Purich D.L. 2010. Enzyme Kinetics: Catalysis and Control: A Reference of
Theory and Best-Practice Methods. Elsevier Science, 32 Jamestown road,
London, UK. 920 pages.
18.Ritz, C.W., Hulet, R.M., Self, B.B., and Denbow, D.M. 1995. “Growth and
intestinal morphology of male turkeys as influenced by dietary supplementation
of amylase and xylanase.” Poultry science. 74(8): 1329–1334.
19.Steel, JW, Wardhaugh, KG. 2002. Ecological impact of macro-cyclic lactones
on dung fauna. In: Vercruysse J, Rew RS (eds) Macrocyclic lactones and antiparasitic therapy. CAB International, Wallingford, Pp: 141–162.
20.Suarez, V.H. 2002. Helminthic control on grazing ruminants and environmental
risks in South America. Veterinary Research, 33: 563–573.
21.Tišler, T., and Eržen, N.K. 2006. Abamectin in the aquatic environment. Journal
of Ecotoxicology, 15: 495-502.
22.Woynarovich, A., Bueno, P.B., Altan, O., Jeney, Zs., Reantaso, M., Xinhua, Y.,
and Van Anrooy, R. 2011. Better management practices for carp production in
central and eastern Europe, the Caucasus and central Asia. FAO Fisheries and
Aquaculture Technical, No. 566. Ankara, FAO. 2011. 153p.
23.Zaghari, M., Majdeddin, M., Taherkhani, R., and Moravej, H. 2008. Estimation
of nutrient equivalency values of natuzyme and its effects on broiler chick
performance. Journal Applied Poultry Research, 17: 446-453.
24.Zamini, A.A., Kanani, H., Esmaeili, A., Ramezani, S., and Zorie Zahara, S. 2012.
“Effects of two dietary exogenous multi-enzyme supplementation, Natuzyme®
and beta-mannanase (Hemicell®), on growth and blood parameters of Caspian
salmon (Salmo trutta caspius).” Comparative Clinical Pathology: 1–6.